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Protein

GBC37740.1

Length

435 amino acids

Protein family membership

None predicted.

Domains and repeats

Domain
50 - 421
THIF-type NAD/FAD binding fold (IPR000594)
Domain
350 - 435
E2 binding (IPR014929)

1 50 100 150 200 250 300 350 400 435

Detailed signature matches

IPR000594

THIF-type NAD/FAD binding fold

PF00899 (ThiF)

51 - 340

Pfam
PF00899 (ThiF)
SSF69572 (Activatin...)

50 - 421

SUPERFAMILY
SSF69572 (Activating enzymes of the ubiquitin-like proteins)

IPR014929

E2 binding

PF08825 (E2_bind)

351 - 431

Pfam
PF08825 (E2_bind)
SM01181 (E2_bind_2)

350 - 435

SMART
SM01181 (E2_bind_2)

IPR033127

Ubiquitin-activating enzyme E1, Cys active site

PS00865 (UBIQUITIN_...)

214 - 222

PROSITE patterns
PS00865 (UBIQUITIN_ACTIVAT_2)

no IPR

Unintegrated signatures

cd01488 (Uba3_RUB)

55 - 344

CDD
cd01488 (Uba3_RUB)

Residue annotation

putative ATP bindi...

60G

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

62G

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

64L

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

84D

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

86D

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

95R

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

108K

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

149G

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)

155A

putative ATP binding site
60G, 62G, 64L, 84D, 86D, 95R, 108K, 149G, 155A
CDD
cd01488 (Uba3_RUB)
substrate interfac...

62G

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

64L

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

150L

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

151D

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

156R

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

182T

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

183E

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

187G

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

188Q

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

202C

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

205D

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

208S

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

209K

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

318Y

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

320M

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

322T

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

328Y

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

330Y

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

332F

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

333E

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)

335Q

substrate interface
62G, 64L, 150L, 151D, 156R, 182T, 183E, 187G, 188Q, 202C, 205D, 208S, 209K, 318Y, 320M, 322T, 328Y, 330Y, 332F, 333E, 335Q
CDD
cd01488 (Uba3_RUB)
dimer interaction ...

67E

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

70K

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

74L

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

91S

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

93L

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

94N

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

95R

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

97F

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

98L

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

100R

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

115F

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

116I

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

118K

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

119R

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

185F

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

276S

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

279Q

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

280G

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

285I

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

286I

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

287P

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

288A

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

289I

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

290A

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)

294A

dimer interaction
67E, 70K, 74L, 91S, 93L, 94N, 95R, 97F, 98L, 100R, 115F, 116I, 118K, 119R, 185F, 276S, 279Q, 280G, 285I, 286I, 287P, 288A, 289I, 290A, 294A
CDD
cd01488 (Uba3_RUB)
zinc binding site ...

199C

zinc binding site
199C, 202C, 340C, 343C
CDD
cd01488 (Uba3_RUB)

202C

zinc binding site
199C, 202C, 340C, 343C
CDD
cd01488 (Uba3_RUB)

340C

zinc binding site
199C, 202C, 340C, 343C
CDD
cd01488 (Uba3_RUB)

343C

zinc binding site
199C, 202C, 340C, 343C
CDD
cd01488 (Uba3_RUB)
catalytic residue ...

216C

catalytic residue
216C
CDD
cd01488 (Uba3_RUB)

GO term prediction

Biological Process

GO:0045116 protein neddylation

Molecular Function

GO:0005524 ATP binding
GO:0008641 small protein activating enzyme activity
GO:0016881 acid-amino acid ligase activity

Cellular Component